iFluor™ 594 Conjugated Cytokeratin 16 Recombinant Rabbit Monoclonal Antibody [SC52-09]
cat.: HA720114F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SC52-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Cytokeratin 16 aa 27-75. |
| Positive control: | A431, human esophagus tissue, human skin tissue. |
| Subcellular location: | Cytoskeleton, Nucleus. |
| Recommended Dilutions:
IF-Cell IF-Tissue |
1:50 1:50 |
| Uniprot #: | SwissProt: P08779 Human |
| Alternative names: | CK 16 CK-16 CK16 Cytokeratin-16 Cytokeratin16 FNEPPK Focal non epidermolytic palmoplantar keratoderma K 16 K16 K1C16_HUMAN K1CP Keratin 1 type I Keratin 16 Keratin Keratin type I cytoskeletal 16 Keratin-16 Keratin16 KRT 16 Krt16 KRT16A NEPPK PC1 type I cytoskeletal 16 |
Images
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Fig1:
Immunocytochemistry analysis of A431 cells labeling Cytokeratin 16. Cells were fixed in methanol and then blocked with 2% negative goat serum for 15 minutes at room temperature. The cells were then incubated overnight at +4℃ with HA720114F at 1/50 dilution Rabbit monoclonal to Cytokeratin 16 (iFluor™ 594)(shown in red). DAPI was used as nuclear counterstain. |
|
Fig2:
Immunofluorescence analysis of paraffin-embedded human esophagus tissue labeling Cytokeratin 16. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. The section was then incubated overnight at +4℃ with HA720114F Cytokeratin 16 (iFluor™ 594, red) at 1/50 dilution, washed with PBS. DAPI was used as nuclear counterstain. |
|
Fig3:
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 16. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. The section was then incubated overnight at +4℃ with HA720114F Cytokeratin 16 (iFluor™ 594, red) at 1/100 dilution, washed with PBS. DAPI was used as nuclear counterstain. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.