iFluor™ 594 Conjugated Cytokeratin 17 Recombinant Rabbit Monoclonal Antibody [SR45-06]
cat.: HA720116F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IF-Tissue, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | SR45-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cytokeratin 17 aa 1-50 / 432. |
| Positive control: | Human skin tissue, MCF-7. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
IF-Tissue IF-Cell |
1:100 1:50 |
| Uniprot #: | SwissProt: Q04695 Human |
| Alternative names: | 39.1 CK 17 CK17 CK-17 Cytokeratin-17 K17 K1C17_HUMAN Keratin 17 keratin 17 epitope S1 keratin 17 epitope S2 keratin 17 epitope S4 Keratin 17, type I Keratin Keratin type I cytoskeletal 17 keratin, type i cytoskeletal 17 [version 1] Keratin-17 KRT17 PC PC2 PCHC1 type I cytoskeletal 17 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 17. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. The section was then incubated overnight at +4℃ with HA720116F Cytokeratin 17 (iFluor™ 594, red) at 1/100 dilution, washed with PBS. DAPI was used as nuclear counterstain. |
|
Fig2:
Immunocytochemistry analysis of MCF-7 cells labeling Cytokeratin 17. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS for 10 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. The cells were then incubated overnight at +4℃ with HA720116F at 1/50 dilution Rabbit monoclonal to Cytokeratin 17 (iFluor™ 594)(shown in red). DAPI was used as nuclear counterstain. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.