iFluor™ 594 Conjugated Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody [ST05-43]
cat.: HA720119F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | ST05-43 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cytokeratin 10 aa 541-584 / 584. |
| Positive control: | Human skin tissue, rat skin tissue. |
| Subcellular location: | Cytoplasm, secreted. |
| Recommended Dilutions:
IF-Tissue |
1:100-1:400 |
| Uniprot #: | SwissProt: P13645 Human | Q6IFW6 Rat |
| Alternative names: | BCIE BIE CK 10 CK10 CK-10 Cytokeratin-10 EHK K10 K1C10_HUMAN Keratin 10 Keratin Keratin type i cytoskeletal 10 Keratin type I cytoskeletal 59 kDa Keratin-10 Keratin10 KPP KRT10 type I cytoskeletal 10 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 10 (HA720119F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 10 (HA720119F, red) at 1/200 dilution at +4℃ overnight, washed with PBS. Nuclei were counterstained with DAPI (blue). |
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Fig2:
Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 10 (HA720119F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 10 (HA720119F, red) at 1/200 dilution at +4℃ overnight, washed with PBS. Nuclei were counterstained with DAPI (blue). |
|
Fig3:
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 10 (HA720119F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (HA720119F, red) at 1/100 dilution and Vimentin (EM0401, green) at 1/400 dilution at +4℃ overnight, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig4:
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 10 (HA720119F), Cytokeratin 5 (HA720129F) and Cytokeratin 1 (HA720141F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (HA720119F, yellow) at 1/400 dilution, Cytokeratin 5 (HA720129F, green) at 1/100 dilution and Cytokeratin 1 (HA720141F, red) at 1/400 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.