iFluor™ 594 Conjugated Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody [ST50-05]
cat.: HA720120F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IF-Cell, IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | ST50-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 51 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cytokeratin 7 aa 18-67. |
| Positive control: | Human breast tissue, human liver tissue, SK-Br-3. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
IF-Cell IF-Tissue FC |
1:100 1:200-1:500 1ug/mL |
| Uniprot #: | SwissProt: P08729 Human | Q9DCV7 Mouse |
| Alternative names: | CK 7 CK-7 CK7 Cytokeratin 7 Cytokeratin-7 D15Wsu77e K2C7 K2C7_HUMAN K7 Keratin 7 Keratin 7, type II Keratin type II cytoskeletal 7 Keratin, 55K type II cytoskeletal Keratin, simple epithelial Keratin, simple epithelial type I, K7 Keratin, type II cytoskeletal 7 Keratin-7 Krt2-7 KRT7 MGC11625 MGC129731 MGC3625 Sarcolectin SCL Type II mesothelial keratin K7 Type-II keratin Kb7 Cytokeratin7 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 7 (HA720120F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 7 (HA720120F, iFluor™ 594) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
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Fig2:
Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 7 (HA720120F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (HA720120F, red) at 1/200 dilution and Vimentin (EM0401, green) at 1/500 dilution at +4℃ overnight, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig3:
Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 7 (HA720120F). Cells were fixed in methanol and then blocked with 2% negative goat serum for 15 minutes at room temperature. The cells were then incubated overnight at +4℃ with Cytokeratin 7 (HA720120F, red) at 1/100 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig4:
Flow cytometric analysis of SK-Br-3 cells labeling Cytokeratin 7. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 7 (HA720120F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.