iFluor™ 594 Conjugated Cytokeratin 15 Recombinant Rabbit Monoclonal Antibody [ST04-85]
cat.: HA720122F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | ST04-85 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 49 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cytokeratin 15 aa 410-455. |
| Positive control: | Human skin tissue, rat skin tissue. |
| Subcellular location: | Intermediate filament, cytosol, extracellular exosome, nucleus. |
| Recommended Dilutions:
IF-Tissue |
1:100-1:400 |
| Uniprot #: | SwissProt: P19012 Human | Q61414 Mouse | Q6IFV3 Rat |
| Alternative names: | AI528832 CK 15 CK-15 CK15 Cytokeratin-15 Cytokeratin15 K15 K1C15_HUMAN K1CO Ka15 Keratin 15 Keratin 15 basic Keratin 15 beta Keratin Keratin complex 1 acidic gene 15 Keratin type I cytoskeletal 15 Keratin-15 Keratin15 KRT 15 Krt1-15 KRT15 KRTB KRTL15 Type I cytoskeletal 15 Type I keratin Ka15 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 15 (HA720122F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (HA720122F, red) at 1/200 dilution at +4℃ overnight, washed with PBS. Nuclei were counterstained with DAPI (blue). |
|
Fig2:
Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 15 (HA720122F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (HA720122F, red) at 1/200 dilution at +4℃ overnight, washed with PBS. Nuclei were counterstained with DAPI (blue). |
|
Fig3:
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 15 (HA720122F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (HA720122F, red) at 1/100 dilution and Vimentin (EM0401, green) at 1/400 dilution at +4℃ overnight, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
|
Fig4:
Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 15 (HA720122F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (HA720122F, red) at 1/100 dilution and Vimentin (EM0401, green) at 1/400 dilution at +4℃ overnight, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.