iFluor™ 594 Conjugated Cytokeratin 4 Recombinant Rabbit Monoclonal Antibody [SN74-03]
cat.: HA720123F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SN74-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 57 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Cytokeratin 4 aa 395-520. |
| Positive control: | Human tonsil tissue. |
| Subcellular location: | Cell surface, intermediate filament cytoskeleton, nucleus. |
| Recommended Dilutions:
IF-Tissue |
1:50-1:100 |
| Uniprot #: | SwissProt: P19013 Human |
| Alternative names: | CK 4 CK-4 CK4 CYK4 Cytokeratin 4 Cytokeratin-4 Cytokeratin4 FLJ31692 K2C4_HUMAN K4 Keratin 4 Keratin Keratin type II cytoskeletal 4 Keratin-4 Keratin4 KRT 4 Krt4 type II cytoskeletal 4 Type-II keratin Kb4 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling Cytokeratin 4 (HA720123F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 4 (HA720123F, red) at 1/50 dilution and Vimentin (EM0401, green) at 1/200 dilution at +4℃ overnight, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.