iFluor™ 594 Conjugated S100 beta Recombinant Rabbit Monoclonal Antibody [SC57-02]
cat.: HA720125F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SC57-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 11 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human S100 beta. |
| Positive control: | human malignant melanoma tissue, human meningioma tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
IF-Tissue |
1:50-1:200 |
| Uniprot #: | SwissProt: P04271 Human | P50114 Mouse | P04631 Rat |
| Alternative names: | NEF Protein S100 B Protein S100-B S 100 calcium binding protein beta chain S 100 protein beta chain S-100 protein beta chain S-100 protein subunit beta S100 S100 calcium binding protein beta (neural) S100 calcium-binding protein B S100 protein beta chain S100B S100B_HUMAN S100beta |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human malignant melanoma tissue labeling S100 beta. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. The section was then incubated overnight at +4℃ with HA720125F S100 beta (iFluor™ 594, red) at 1/100 dilution, washed with PBS. DAPI was used as nuclear counterstain. |
|
Fig2:
Immunofluorescence analysis of paraffin-embedded human meningioma tissue labeling S100 beta. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. The section was then incubated overnight at +4℃ with HA720125F S100 beta (iFluor™ 594, red) at 1/100 dilution, washed with PBS. DAPI was used as nuclear counterstain. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.