iFluor™ 488 Conjugated Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody [SC62-04]
cat.: HA720129F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Tissue, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | SC62-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Cytokeratin 5 aa 70-110. |
| Positive control: | Human skin tissue, A431. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
IF-Tissue IF-Cell |
1:100-1:200 1:100 |
| Uniprot #: | SwissProt: P13647 Human |
| Alternative names: | 58 kDa cytokeratin CK-5 CK5 Cytokeratin-5 Cytokeratin5 DDD DDD1 EBS2 epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types K2C5_HUMAN K5 keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) Keratin 5 Keratin keratin complex 2, basic, gene 5 keratin, type II cytoskeletal 5 Keratin-5 Keratin5 KRT 5 Krt5 KRT5A type II cytoskeletal 5 Type-II keratin Kb5 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 5 (HA720129F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 5 (HA720129F, green) at 1/200 dilution and Vimentin (EM0401, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
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Fig2:
Immunocytochemistry analysis of A431 cells labeling Cytokeratin 5 with Rabbit anti-Cytokeratin 5 antibody (HA720129F) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 5 antibody (HA720129F) at 1/100 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/800 dilution. |
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Fig3:
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 5 (HA720129F), Cytokeratin 1 (HA720141F) and Cytokeratin 10 (HA720119F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 5 (HA720129F, green) at 1/100 dilution, Cytokeratin 1 (HA720141F, red) at 1/400 dilution and Cytokeratin 10 (HA720119F, yellow) at 1/400 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.