iFluor™ 488 Conjugated Cytokeratin 6 Recombinant Rabbit Monoclonal Antibody [SN71-07]
cat.: HA720130F
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: IF-Cell
Clonality: Monoclonal
Clone number: SN71-07
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 60 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Cytokeratin 6 aa 1-118 / 564.
Positive control: A431.
Subcellular location: Keratin filament, cytosol, extracellular exosome, nucleus, membrane.
Recommended Dilutions:
  IF-Cell

1:100
Uniprot #: SwissProt: P02538 Human | P04259 Human | P48668 Human
Alternative names: CK6 CK 6A CK 6B CK 6C CK 6D CK 6E CK-6B CK-6C CK-6E Cytokeratin 6a Cytokeratin 6B Cytokeratin 6C Cytokeratin 6D Cytokeratin 6E Cytokeratin-6B Cytokeratin-6C Cytokeratin-6E K2C6C_HUMAN K6a keratin K6b keratin K6C K6c keratin K6d keratin K6e keratin Keratin Keratin K6h Keratin type II cytoskeletal 6A Keratin type II cytoskeletal 6B Keratin type II cytoskeletal 6C Keratin type II cytoskeletal 6D Keratin type II cytoskeletal 6E Keratin-6C KRT6A KRT6B KRT6C KRT6D KRT6E type II cytoskeletal 6C Type-II keratin Kb12
Images
HA720130F_1.jpg Fig1: Immunocytochemistry analysis of A431 cells labeling Cytokeratin 6 with Rabbit anti-Cytokeratin 6 antibody (HA720130F) at 1/100 dilution.

Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 6 antibody (HA720130F) at 1/100 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.