iFluor™ 488 Conjugated Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody [SU0338]
cat.: HA720132F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Tissue, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | SU0338 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cytokeratin 8 aa 321-370 / 483. |
| Positive control: | Human liver tissue, SK-Br-3, human breast tissue. |
| Subcellular location: | Nucleoplasm, Nucleus matrix, Cytoplasm. |
| Recommended Dilutions:
IF-Tissue IF-Cell |
1:200 1:100 |
| Uniprot #: | SwissProt: P05787 Human |
| Alternative names: | CARD2 Cell proliferation inducing gene 46 protein Cell proliferation inducing protein 46 CK 8 CK-8 CK8 CYK8 Cytokeratin 8 Cytokeratin-8 K2C8 K2C8_HUMAN K8 Keratin 8 Keratin keratin type II cytoskeletal 8 Keratin-8 KRT8 type II cytoskeletal 8 Type-II keratin Kb8 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 8 (HA720132F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (HA720132F, green) at 1/200 dilution and Vimentin (EM0401, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
|
Fig2:
Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 8 with Rabbit anti-Cytokeratin 8 antibody (HA720132F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 8 antibody (HA720132F) at 1/100 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/800 dilution. |
|
Fig3:
Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 8 (HA720132F) and Cytokeratin 7 (HA720144F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (HA720132F, green) at 1/200 dilution and Cytokeratin 7 (HA720144F, red) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
|
Fig4:
Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 8 (HA720132F), Cytokeratin 7 (HA720144F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (HA720132F, green) at 1/200 dilution, Cytokeratin 7 (HA720144F, red) at 1/50 dilution and Vimentin (EM0401, yellow) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Alexa Fluor® 555 conjugate-Goat anti-Mouse IgG (HA1125) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.