iFluor™ 488 Conjugated Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody [SU0338]
cat.: HA720132F
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: IF-Tissue, IF-Cell
Clonality: Monoclonal
Clone number: SU0338
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 54 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Cytokeratin 8 aa 321-370 / 483.
Positive control: Human liver tissue, SK-Br-3, human breast tissue.
Subcellular location: Nucleoplasm, Nucleus matrix, Cytoplasm.
Recommended Dilutions:
  IF-Tissue
  IF-Cell

1:200
1:100
Uniprot #: SwissProt: P05787 Human
Alternative names: CARD2 Cell proliferation inducing gene 46 protein Cell proliferation inducing protein 46 CK 8 CK-8 CK8 CYK8 Cytokeratin 8 Cytokeratin-8 K2C8 K2C8_HUMAN K8 Keratin 8 Keratin keratin type II cytoskeletal 8 Keratin-8 KRT8 type II cytoskeletal 8 Type-II keratin Kb8
Images
HA720132F_1.jpg Fig1: Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 8 (HA720132F) and Vimentin (EM0401).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (HA720132F, green) at 1/200 dilution and Vimentin (EM0401, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.

iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.
HA720132F_2.jpg Fig2: Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 8 with Rabbit anti-Cytokeratin 8 antibody (HA720132F) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 8 antibody (HA720132F) at 1/100 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/800 dilution.
HA720132F_3.jpg Fig3: Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 8 (HA720132F) and Cytokeratin 7 (HA720144F).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (HA720132F, green) at 1/200 dilution and Cytokeratin 7 (HA720144F, red) at 1/200 dilution overnight at 4 ℃, washed with PBS.

DAPI was used as nuclear counterstain.
HA720132F_4.jpg Fig4: Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 8 (HA720132F), Cytokeratin 7 (HA720144F) and Vimentin (EM0401).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (HA720132F, green) at 1/200 dilution, Cytokeratin 7 (HA720144F, red) at 1/50 dilution and Vimentin (EM0401, yellow) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.

Alexa Fluor® 555 conjugate-Goat anti-Mouse IgG (HA1125) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.