iFluor™ 488 Conjugated Cytokeratin 9 Recombinant Rabbit Monoclonal Antibody [SD201-09]
cat.: HA720133F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SD201-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein wiehin Human Cytokeratin 9 aa 234-422 / 623. |
| Positive control: | Rat skin tissue. |
| Subcellular location: | Extracellular exosome, membrane, nucleus. |
| Recommended Dilutions:
IF-Tissue |
1:200 |
| Uniprot #: | SwissProt: P35527 Human | Q6RHW0 Mouse |
| Alternative names: | CK 9 CK9 CK-9 Cytokeratin 9 Cytokeratin-9 Cytokeratin9 EPPK K1C9_HUMAN K9 Keratin 9 Keratin Keratin type I cytoskeletal 9 Keratin-9 Keratin9 KRT 9 Krt9 Spermatid Perinuclear Ring Manchette Protein K9 type I cytoskeletal 9 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 9 (HA720133F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 9 (HA720133F, green) at 1/200 dilution and Vimentin (EM0401, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.