iFluor™ 488 Conjugated Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody [ST05-43]
cat.: HA720134F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | ST05-43 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cytokeratin 10 aa 541-584 / 584. |
| Positive control: | Human skin tissue, rat skin tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
IF-Tissue |
1:400-1:500 |
| Uniprot #: | SwissProt: P13645 Human | Q6IFW6 Rat |
| Alternative names: | BCIE BIE CK 10 CK10 CK-10 Cytokeratin-10 EHK K10 K1C10_HUMAN Keratin 10 Keratin Keratin type i cytoskeletal 10 Keratin type I cytoskeletal 59 kDa Keratin-10 Keratin10 KPP KRT10 type I cytoskeletal 10 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 10 (HA720134F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (HA720134F, green) at 1/500 dilution and Vimentin (EM0401, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
|
Fig2:
Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 10 (HA720134F), Cytokeratin 14 (HA720147F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (HA720134F, green) at 1/400 dilution, Cytokeratin 14 (HA720147F, red) at 1/400 dilution and Vimentin (EM0401, yellow) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Alexa Fluor® 555 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.