iFluor™ 488 Conjugated Cytokeratin 14 Recombinant Rabbit Monoclonal Antibody [SC65-06]
cat.: HA720135F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | IF-Tissue, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | SC65-06 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Cytokeratin 14 aa 250-484. |
| Positive control: | Rat skin tissue, mouse skin tissue, B16F1. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
IF-Tissue IF-Cell |
1:100-1:500 1:100 |
| Uniprot #: | SwissProt: P02533 Human | Q61781 Mouse | Q6IFV1 Rat |
| Alternative names: | CK 14 CK-14 ck14 Cytokeratin 14 Cytokeratin-14 Cytokeratin14 Dowling Meara EBS3 EBS4 Epidermolysis bullosa simplex K14 K1C14_HUMAN Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner) Keratin 14 Keratin Keratin type I cytoskeletal 14 Keratin, type I cytoskeletal 14 Keratin-14 Keratin14 Koebner Krt 14 Krt14 NFJ OTTHUMP00000164624 type I cytoskeletal 14 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 14 (HA720135F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 14 (HA720135F, green) at 1/100 dilution and Vimentin (EM0401, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
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Fig2:
Immunofluorescence analysis of paraffin-embedded mouse skin tissue labeling Cytokeratin 14 with Rabbit anti-Cytokeratin 14 antibody (HA720135F) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720135F, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue). |
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Fig3:
Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 14 with Rabbit anti-Cytokeratin 14 antibody (HA720135F) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720135F, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue). |
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Fig4:
Immunocytochemistry analysis of B16F1 cells labeling Cytokeratin 14 with Rabbit anti-Cytokeratin 14 antibody (HA720135F) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 14 antibody (HA720135F) at 1/100 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.