iFluor™ 488 Conjugated Cytokeratin 15 Recombinant Rabbit Monoclonal Antibody [ST04-85]
cat.: HA720136F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Tissue, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | ST04-85 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 49 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cytokeratin 15 aa 410-455. |
| Positive control: | Human breast tissue, MCF-7, human skin tissue, rat skin tissue, mouse skin tissue, A431. |
| Subcellular location: | Intermediate filament, cytosol, extracellular exosome, nucleus. |
| Recommended Dilutions:
IF-Tissue IF-Cell FC |
1:200-1:400 1:50-1:100 1ug/mL |
| Uniprot #: | SwissProt: P19012 Human | Q61414 Mouse | Q6IFV3 Rat |
| Alternative names: | AI528832 CK 15 CK-15 CK15 Cytokeratin-15 Cytokeratin15 K15 K1C15_HUMAN K1CO Ka15 Keratin 15 Keratin 15 basic Keratin 15 beta Keratin Keratin complex 1 acidic gene 15 Keratin type I cytoskeletal 15 Keratin-15 Keratin15 KRT 15 Krt1-15 KRT15 KRTB KRTL15 Type I cytoskeletal 15 Type I keratin Ka15 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 15 (HA720136F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (HA720136F, green) at 1/400 dilution and Vimentin (EM0401, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
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Fig2:
Immunocytochemistry analysis of MCF-7 cells labeling Cytokeratin 15 with Rabbit anti-Cytokeratin 15 antibody (HA720136F) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 15 antibody (HA720136F) at 1/100 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution. |
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Fig3:
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 15 (HA720136F), Cytokeratin 10 (HA720146F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (HA720136F, green) at 1/200 dilution, Cytokeratin 10 (HA720146F, red) at 1/50 dilution and Vimentin (EM0401, magenta) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Alexa Fluor® 555 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
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Fig4:
Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 15 (HA720136F), Cytokeratin 10 (HA720146F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (HA720136F, green) at 1/200 dilution, Cytokeratin 10 (HA720146F, red) at 1/50 dilution and Vimentin (EM0401, magenta) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Alexa Fluor® 555 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
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Fig5:
Immunofluorescence analysis of paraffin-embedded mouse skin tissue labeling Cytokeratin 15 (HA720136F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 15 (HA720136F, iFluor™ 488) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
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Fig6:
Immunocytochemistry analysis of A431 cells labeling Cytokeratin 15 with Rabbit anti-Cytokeratin 15 antibody (HA720136F) at 1/50 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 15 antibody (HA720136F) at 1/50 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. |
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Fig7:
Flow cytometric analysis of A431 cells labeling Cytokeratin 15. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 15 (HA720136F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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