iFluor™ 488 Conjugated Cytokeratin 17 Recombinant Rabbit Monoclonal Antibody [SR45-06]
cat.: HA720138F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IF-Tissue, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | SR45-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cytokeratin 17 aa 1-50 / 432. |
| Positive control: | Human esophagus tissue, Hela. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
IF-Tissue IF-Cell |
1:200 1:100 |
| Uniprot #: | SwissProt: Q04695 Human | Q9QWL7 Mouse |
| Alternative names: | 39.1 CK 17 CK17 CK-17 Cytokeratin-17 K17 K1C17_HUMAN Keratin 17 keratin 17 epitope S1 keratin 17 epitope S2 keratin 17 epitope S4 Keratin 17, type I Keratin Keratin type I cytoskeletal 17 keratin, type i cytoskeletal 17 [version 1] Keratin-17 KRT17 PC PC2 PCHC1 type I cytoskeletal 17 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human esophagus tissue labeling Cytokeratin 17 (HA720138F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 17 (HA720138F, green) at 1/200 dilution and Vimentin (EM0401, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
|
Fig2:
Immunocytochemistry analysis of Hela cells labeling Cytokeratin 17 with Rabbit anti-Cytokeratin 17 antibody (HA720138F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 17 antibody (HA720138F) at 1/100 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.