iFluor™ 647 Conjugated Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody [SN72-08]
cat.: HA720141F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SN72-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 66 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse Cytokeratin 1 aa 586-637. |
| Positive control: | Human skin tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IF-Tissue |
1:100-1:400 |
| Uniprot #: | SwissProt: P04264 Human | P04104 Mouse | Q6IMF3 Rat |
| Alternative names: | 67 kDa cytokeratin CK-1 CK1 Cytokeratin-1 Cytokeratin1 EHK EHK1 Epidermolytic hyperkeratosis 1 EPPK Hair alpha protein K1 K2C1_HUMAN Keratin Keratin type II cytoskeletal 1 Keratin-1 Keratin1 KRT 1 Krt1 KRT1A NEPPK type II cytoskeletal 1 Type II keratin Kb1 Type-II keratin Kb1 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1 (HA720141F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 1 (HA720141F, red) at 1/100 dilution and Vimentin (EM0401, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
|
Fig2:
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1 (HA720141F), Cytokeratin 5 (HA720129F) and Cytokeratin 10 (HA720119F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 1 (HA720141F, red) at 1/400 dilution, Cytokeratin 5 (HA720129F, green) at 1/100 dilution and Cytokeratin 10 (HA720119F, yellow) at 1/400 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.