iFluor™ 647 Conjugated Cytokeratin 4 Recombinant Rabbit Monoclonal Antibody [SN74-03]
cat.: HA720142F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SN74-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Cytokeratin 4 aa 395-520. |
| Positive control: | A431, human tonsil tissue. |
| Subcellular location: | Cell surface, intermediate filament cytoskeleton, nucleus. |
| Recommended Dilutions:
IF-Cell IF-Tissue |
1:100 1:200 |
| Uniprot #: | SwissProt: P19013 Human |
| Alternative names: | CK 4 CK-4 CK4 CYK4 Cytokeratin 4 Cytokeratin-4 Cytokeratin4 FLJ31692 K2C4_HUMAN K4 Keratin 4 Keratin Keratin type II cytoskeletal 4 Keratin-4 Keratin4 KRT 4 Krt4 type II cytoskeletal 4 Type-II keratin Kb4 |
Images
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Fig1:
Immunocytochemistry analysis of A431 cells labeling Cytokeratin 4 with Rabbit anti-Cytokeratin 4 antibody (HA720142F) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37℃. Cells were then incubated with Rabbit anti-Cytokeratin 4 antibody (HA720142F, red) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/800 dilution. |
|
Fig2:
Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling Cytokeratin 4 (HA720142F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 4 (HA720142F, red) at 1/200 dilution and Vimentin (EM0401, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.