iFluor™ 647 Conjugated Cytokeratin 6 Recombinant Rabbit Monoclonal Antibody [SN71-07]
cat.: HA720143F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SN71-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Cytokeratin 6 aa 1-118 / 564. |
| Positive control: | A431, human tonsil tissue. |
| Recommended Dilutions:
IF-Cell IF-Tissue |
1:100 1:100 |
| Uniprot #: | SwissProt: P02538 Human | P04259 Human | P48668 Human | Q4FZU2 Rat |
| Alternative names: | CK6 CK 6A CK 6B CK 6C CK 6D CK 6E CK-6B CK-6C CK-6E Cytokeratin 6a Cytokeratin 6B Cytokeratin 6C Cytokeratin 6D Cytokeratin 6E Cytokeratin-6B Cytokeratin-6C Cytokeratin-6E K2C6C_HUMAN K6a keratin K6b keratin K6C K6c keratin K6d keratin K6e keratin Keratin Keratin K6h Keratin type II cytoskeletal 6A Keratin type II cytoskeletal 6B Keratin type II cytoskeletal 6C Keratin type II cytoskeletal 6D Keratin type II cytoskeletal 6E Keratin-6C KRT6A KRT6B KRT6C KRT6D KRT6E type II cytoskeletal 6C Type-II keratin Kb12 |
Images
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Fig1:
Immunocytochemistry analysis of A431 cells labeling Cytokeratin 6 with Rabbit anti-Cytokeratin 6 antibody (HA720143F) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37℃. Cells were then incubated with Rabbit anti-Cytokeratin 6 antibody (HA720143F, red) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/800 dilution. |
|
Fig2:
Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling Cytokeratin 6 (HA720143F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 6 (HA720143F, red) at 1/100 dilution and Vimentin (EM0401, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.