iFluor™ 647 Conjugated Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody [ST50-05]
cat.: HA720144F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IF-Cell, IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | ST50-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cytokeratin 7 aa 18-67. |
| Positive control: | SK-Br-3, human liver tissue, human breast tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
IF-Cell IF-Tissue FC |
1:100 1:50-1:200 1ug/mL |
| Uniprot #: | SwissProt: P08729 Human | Q9DCV7 Mouse |
| Alternative names: | CK 7 CK-7 CK7 Cytokeratin 7 Cytokeratin-7 D15Wsu77e K2C7 K2C7_HUMAN K7 Keratin 7 Keratin 7, type II Keratin type II cytoskeletal 7 Keratin, 55K type II cytoskeletal Keratin, simple epithelial Keratin, simple epithelial type I, K7 Keratin, type II cytoskeletal 7 Keratin-7 Krt2-7 KRT7 MGC11625 MGC129731 MGC3625 Sarcolectin SCL Type II mesothelial keratin K7 Type-II keratin Kb7 Cytokeratin7 |
Images
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Fig1:
Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 7 with Rabbit anti-Cytokeratin 7 antibody (HA720144F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37℃. Cells were then incubated with Rabbit anti-Cytokeratin 7 antibody (HA720144F, red) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta III Tubulin (M0805-8, green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/800 dilution. |
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Fig2:
Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 7 (HA720144F) and Cytokeratin 8 (HA720132F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (HA720144F, red) at 1/200 dilution and Cytokeratin 8 (HA720132F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
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Fig3:
Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 7 (HA720144F), Cytokeratin 8 (HA720132F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (HA720144F, red) at 1/50 dilution, Cytokeratin 8 (HA720132F, green) at 1/200 dilution and Vimentin (EM0401, yellow) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Alexa Fluor® 555 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
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Fig4:
Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 7 (HA720144F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 7 (HA720144F, iFluor™ 647) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
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Fig5:
Flow cytometric analysis of SK-Br-3 cells labeling Cytokeratin 7. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 7 (HA720144F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.