iFluor™ 647 Conjugated Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody [SU0338]
cat.: HA720145F
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: IF-Cell, IF-Tissue
Clonality: Monoclonal
Clone number: SU0338
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 54 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Cytokeratin 8 aa 321-370 / 483.
Positive control: SK-Br-3, human breast tissue.
Subcellular location: Nucleoplasm, Nucleus matrix, Cytoplasm.
Recommended Dilutions:
  IF-Cell
  IF-Tissue

1:100
1:200
Uniprot #: SwissProt: P05787 Human | P11679 Mouse
Alternative names: CARD2 Cell proliferation inducing gene 46 protein Cell proliferation inducing protein 46 CK 8 CK-8 CK8 CYK8 Cytokeratin 8 Cytokeratin-8 K2C8 K2C8_HUMAN K8 Keratin 8 Keratin keratin type II cytoskeletal 8 Keratin-8 KRT8 type II cytoskeletal 8 Type-II keratin Kb8
Images
HA720145F_1.jpg Fig1: Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 8 with Rabbit anti-Cytokeratin 8 antibody (HA720145F) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37℃. Cells were then incubated with Rabbit anti-Cytokeratin 8 antibody (HA720145F, red) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

Beta III Tubulin (M0805-8, green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/800 dilution.
HA720145F_2.jpg Fig2: Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 8 (HA720145F) and Vimentin (EM0401).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (HA720145F, red) at 1/200 dilution and Vimentin (EM0401, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.

iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.