iFluor™ 647 Conjugated Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody [SU0338]
cat.: HA720145F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IF-Tissue, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | SU0338 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cytokeratin 8 aa 321-370 / 483. |
| Positive control: | Human breast tissue, SK-Br-3. |
| Subcellular location: | Nucleoplasm, Nucleus matrix, Cytoplasm. |
| Recommended Dilutions:
IF-Tissue IF-Cell FC |
1:200 1:100 1:100 |
| Uniprot #: | SwissProt: P05787 Human | P11679 Mouse |
| Alternative names: | CARD2 Cell proliferation inducing gene 46 protein Cell proliferation inducing protein 46 CK 8 CK-8 CK8 CYK8 Cytokeratin 8 Cytokeratin-8 K2C8 K2C8_HUMAN K8 Keratin 8 Keratin keratin type II cytoskeletal 8 Keratin-8 KRT8 type II cytoskeletal 8 Type-II keratin Kb8 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 8 (HA720145F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (HA720145F, red) at 1/200 dilution and Vimentin (EM0401, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
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Fig2:
Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 8 with Rabbit anti-Cytokeratin 8 antibody (HA720145F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37℃. Cells were then incubated with Rabbit anti-Cytokeratin 8 antibody (HA720145F, red) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta III Tubulin (M0805-8, green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/800 dilution. |
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Fig3:
Flow cytometric analysis of SK-Br-3 cells labeling Cytokeratin 8 (HA720145F, iFluor™ 647). Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 8 (HA720145F, red, 1/100) and Rabbit IgG Isotype Control (iFluor™ 647, green, 1/100). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.