iFluor™ 647 Conjugated Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody [ST05-43]
cat.: HA720146F
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat, Mouse
Applications: IF-Tissue
Clonality: Monoclonal
Clone number: ST05-43
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 59 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Cytokeratin 10 aa 541-584 / 584.
Positive control: Human skin tissue, rat skin tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  IF-Tissue

1:50
Uniprot #: SwissProt: P13645 Human | Q6IFW6 Rat
Alternative names: BCIE BIE CK 10 CK10 CK-10 Cytokeratin-10 EHK K10 K1C10_HUMAN Keratin 10 Keratin Keratin type i cytoskeletal 10 Keratin type I cytoskeletal 59 kDa Keratin-10 Keratin10 KPP KRT10 type I cytoskeletal 10
Images
HA720146F_1.jpg Fig1: Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 10 (HA720146F), Cytokeratin 15 (HA720136F) and Vimentin (EM0401).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (HA720146F, red) at 1/50 dilution, Cytokeratin 15 (HA720136F, green) at 1/200 dilution and Vimentin (EM0401, magenta) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.

Alexa Fluor® 555 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.
HA720146F_2.jpg Fig2: Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 10 (HA720146F), Cytokeratin 15 (HA720136F) and Vimentin (EM0401).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (HA720146F, red) at 1/50 dilution, Cytokeratin 15 (HA720136F, green) at 1/200 dilution and Vimentin (EM0401, magenta) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.

Alexa Fluor® 555 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.