iFluor™ 647 Conjugated Cytokeratin 15 Recombinant Rabbit Monoclonal Antibody [ST04-85]
cat.: HA720148F
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: IF-Tissue
Clonality: Monoclonal
Clone number: ST04-85
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 49 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Cytokeratin 15 aa 410-455.
Positive control: Human skin tissue.
Subcellular location: Intermediate filament, cytosol, extracellular exosome, nucleus.
Recommended Dilutions:
  IF-Tissue

1:200
Uniprot #: SwissProt: P19012 Human | Q61414 Mouse | Q6IFV3 Rat
Alternative names: AI528832 CK 15 CK-15 CK15 Cytokeratin-15 Cytokeratin15 K15 K1C15_HUMAN K1CO Ka15 Keratin 15 Keratin 15 basic Keratin 15 beta Keratin Keratin complex 1 acidic gene 15 Keratin type I cytoskeletal 15 Keratin-15 Keratin15 KRT 15 Krt1-15 KRT15 KRTB KRTL15 Type I cytoskeletal 15 Type I keratin Ka15
Images
HA720148F_1.jpg Fig1: Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 15 (HA720148F) and Vimentin (EM0401).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (HA720148F, red) at 1/200 dilution and Vimentin (EM0401, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.

iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.