iFluor™ 647 Conjugated Cytokeratin 19 Recombinant Rabbit Monoclonal Antibody [SA30-06]
cat.: HA720152F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IF-Cell, IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | SA30-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cytokeratin 19 aa 348-400. |
| Positive control: | SK-Br-3, human liver tissue, human breast tissue, human kidney tissue, MCF-7. |
| Subcellular location: | Cytoskeleton. |
| Recommended Dilutions:
IF-Cell IF-Tissue FC |
1:100 1:50-1:100 1 ug/mL |
| Uniprot #: | SwissProt: P08727 Human | P19001 Mouse |
| Alternative names: | 40 kDa keratin intermediate filament CK 19 CK-19 CK19 Cytokeratin 19 Cytokeratin-19 K19 K1C19_HUMAN K1CS Keratin 19 Keratin type I 40 kD Keratin type I 40kD Keratin type I cytoskeletal 19 Keratin, type I cytoskeletal 19 Keratin, type I, 40 kd Keratin-19 KRT19 MGC15366 |
Images
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Fig1:
Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 19 with Rabbit anti-Cytokeratin 19 antibody (HA720152F) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37℃. Cells were then incubated with Rabbit anti-Cytokeratin 19 antibody (HA720152F, red) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/800 dilution. |
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Fig2:
Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 19 (HA720152F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 19 (HA720152F, red) at 1/50 dilution and Vimentin (EM0401, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
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Fig3:
Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 19 (HA720152F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 19 (HA720152F, iFluor™ 647) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
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Fig4:
Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Cytokeratin 19 (HA720152F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 19 (HA720152F, iFluor™ 647) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
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Fig5:
Flow cytometric analysis of MCF-7 cells labeling Cytokeratin 19. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 19 (HA720152F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6:
Flow cytometric analysis of SK-Br-3 cells labeling Cytokeratin 19. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 19 (HA720152F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.