|Product Type:||Recombinant Rabbit monoclonal IgG, primary antibodies|
Immunocytochemistry analysis of SH-SY5Y cells labeling Calretinin with Rabbit anti-Calretinin antibody (HA720153F) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Calretinin antibody (HA720153F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.
Flow cytometric analysis of SH-SY5Y cells labeling Calretinin.
Cells were fixed and permeabilized. Then incubated for 30 minutes at +4℃ with Calretinin (HA720153F, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).