iFluor™ 488 Conjugated Calretinin Recombinant Rabbit Monoclonal Antibody [JM12-93]
cat.: HA720153F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JM12-93 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Calretinin aa 60-100. |
| Positive control: | SH-SY5Y. |
| Subcellular location: | Cuticular plate, cytosol, nucleus, synaptic membrane, dendrite, gap junction, neuron projection, parallel fiber to Purkinje cell synapse, stereocilium, synapse, terminal bouton. |
| Recommended Dilutions:
IF-Cell FC |
1:100 1:500-1:1,000 |
| Uniprot #: | SwissProt: P22676 Human |
| Alternative names: | 29 kDa calbindin CAB 29 CAB29 CAL 2 CAL2 CALB 2 CALB2 CALB2_HUMAN Calbindin 2 29kDa Calbindin 2 Calbindin D29K Calbindin2 Calretinin CR |
Images
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Fig1:
Immunocytochemistry analysis of SH-SY5Y cells labeling Calretinin with Rabbit anti-Calretinin antibody (HA720153F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Calretinin antibody (HA720153F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution. |
|
Fig2:
Flow cytometric analysis of SH-SY5Y cells labeling Calretinin. Cells were fixed and permeabilized. Then incubated for 30 minutes at +4℃ with Calretinin (HA720153F, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.