iFluor™ 488 Conjugated Glucose Transporter GLUT1 Recombinant Rabbit Monoclonal Antibody [SA0377]
cat.: HA720154F
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: IF-Cell, IF-Tissue
Clonality: Monoclonal
Clone number: SA0377
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 54 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human GLUT1 aa 443-492 / 492.
Positive control: MCF7, human placenta tissue, MDA-MB-468.
Subcellular location: Cell membrane, Melanosome
Recommended Dilutions:
  IF-Cell
  IF-Tissue

1:100
1:50
Uniprot #: SwissProt: P11166 Human | P17809 Mouse | P11167 Rat
Alternative names: Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) CSE DYT17 DYT18 DYT9 EIG12 erythrocyte/brain Erythrocyte/hepatoma glucose transporter facilitated glucose transporter member 1 Glucose transporter 1 Glucose transporter type 1 Glucose transporter type 1, erythrocyte/brain GLUT GLUT-1 GLUT1 GLUT1DS GLUTB GT1 GTG1 Gtg3 GTR1_HUMAN HepG2 glucose transporter HTLVR Human T cell leukemia virus (I and II) receptor MGC141895 MGC141896 PED RATGTG1 Receptor for HTLV 1 and HTLV 2 SLC2A1 Solute carrier family 2 (facilitated glucose transporter), member 1 Solute carrier family 2 Solute carrier family 2, facilitated glucose transporter member 1
Images
HA720154F_1.jpg Fig1: Immunocytochemistry analysis of MCF7 cells labeling Glucose Transporter GLUT1 with Rabbit anti-Glucose Transporter GLUT1 antibody (HA720154F) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody (HA720154F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.
HA720154F_2.jpg Fig2: Immunofluorescence analysis of paraffin-embedded human placenta tissue labeling Glucose Transporter GLUT1 (HA720154F).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Glucose Transporter GLUT1 (HA720154F, iFluor™ 488) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.
HA720154F_3.jpg Fig3: Immunocytochemistry analysis of MDA-MB-468 cells labeling Glucose Transporter GLUT1 with Rabbit anti-Glucose Transporter GLUT1 antibody (HA720154F) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody (HA720154F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.