Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | IF-Cell, IF-Tissue |
Clonality: | Monoclonal |
Clone number: | SA0377 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 54 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human GLUT1 aa 443-492 / 492. |
Positive control: | MCF7, human placenta tissue, MDA-MB-468. |
Subcellular location: | Cell membrane, Melanosome |
Recommended Dilutions:
IF-Cell IF-Tissue |
1:100 1:50 |
Uniprot #: | SwissProt: P11166 Human | P17809 Mouse | P11167 Rat |
Alternative names: | Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) CSE DYT17 DYT18 DYT9 EIG12 erythrocyte/brain Erythrocyte/hepatoma glucose transporter facilitated glucose transporter member 1 Glucose transporter 1 Glucose transporter type 1 Glucose transporter type 1, erythrocyte/brain GLUT GLUT-1 GLUT1 GLUT1DS GLUTB GT1 GTG1 Gtg3 GTR1_HUMAN HepG2 glucose transporter HTLVR Human T cell leukemia virus (I and II) receptor MGC141895 MGC141896 PED RATGTG1 Receptor for HTLV 1 and HTLV 2 SLC2A1 Solute carrier family 2 (facilitated glucose transporter), member 1 Solute carrier family 2 Solute carrier family 2, facilitated glucose transporter member 1 |
Fig1:
Immunocytochemistry analysis of MCF7 cells labeling Glucose Transporter GLUT1 with Rabbit anti-Glucose Transporter GLUT1 antibody (HA720154F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody (HA720154F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution. |
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Fig2:
Immunofluorescence analysis of paraffin-embedded human placenta tissue labeling Glucose Transporter GLUT1 (HA720154F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Glucose Transporter GLUT1 (HA720154F, iFluor™ 488) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
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Fig3:
Immunocytochemistry analysis of MDA-MB-468 cells labeling Glucose Transporter GLUT1 with Rabbit anti-Glucose Transporter GLUT1 antibody (HA720154F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody (HA720154F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution. |