iFluor™ 488 Conjugated Collagen VI Recombinant Rabbit Monoclonal Antibody [SD83-03]
cat.: HA720155F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SD83-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 109 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Collagen VI aa 17-255 / 1,028. |
| Positive control: | Hela, mouse colon tissue. |
| Subcellular location: | Extracellular matrix. |
| Recommended Dilutions:
IF-Cell IF-Tissue |
1:100 1:50 |
| Uniprot #: | SwissProt: P12109 Human | P12110 Human | P12111 Human | Q02788 Mouse | Q04857 Mouse |
| Alternative names: | Alpha 1 (VI) chain (61 AA) CO6A1_HUMAN COL6A1 COL6A2 COL6A3 Collagen alpha 2(VI) chain Collagen alpha 3(VI) chain Collagen alpha-1(VI) chain Collagen type VI alpha 1 Collagen type VI alpha 2 Collagen type VI alpha 3 Collagen VI alpha 1 polypeptide Collagen VI alpha 2 polypeptide Collagen VI alpha 3 polypeptide CollagenVI Human mRNA for collagen VI alpha 2 C terminal globular domain OPLL PP3610 |
Images
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Fig1:
Immunocytochemistry analysis of Hela cells labeling Collagen VI with Rabbit anti-Collagen VI antibody (HA720155F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Collagen VI antibody (HA720155F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution. |
|
Fig2:
Immunofluorescence analysis of paraffin-embedded mouse colon tissue labeling Collagen VI (HA720155F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Collagen VI (HA720155F, iFluor™ 488) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.