iFluor™ 488 Conjugated OGT Recombinant Rabbit Monoclonal Antibody [JB44-39]
cat.: HA720156F
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: IF-Cell
Clonality: Monoclonal
Clone number: JB44-39
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 117 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human OGT aa 997-1,046 / 1,046.
Positive control: Hela.
Subcellular location: Nucleus. Cytoplasm. Membrane.
Recommended Dilutions:
  IF-Cell

1:75
Uniprot #: SwissProt: O15294 Human
Alternative names: FLJ23071 GlcNAc transferase HRNT1 MGC22921 O GlcNAc O GlcNAc transferase p110 subunit O GlcNAc transferase subunit p110 O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) O linked N acetylglucosamine (GlcNAc) transferase O linked N acetylglucosamine transferase 110 kDa subunit O-GlcNAc transferase subunit p110 O-linked N-acetylglucosamine transferase 110 kDa subunit ogt OGT1_HUMAN UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase
Images
HA720156F_1.jpg Fig1: Immunocytochemistry analysis of Hela cells labeling OGT with Rabbit anti-OGT antibody (HA720156F) at 1/75 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-OGT antibody (HA720156F) at 1/75 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.