iFluor™ 488 Conjugated OGT Recombinant Rabbit Monoclonal Antibody [JB44-39]
cat.: HA720156F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JB44-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 117 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human OGT aa 997-1,046 / 1,046. |
| Positive control: | Hela. |
| Subcellular location: | Nucleus. Cytoplasm. Membrane. |
| Recommended Dilutions:
IF-Cell |
1:75 |
| Uniprot #: | SwissProt: O15294 Human |
| Alternative names: | FLJ23071 GlcNAc transferase HRNT1 MGC22921 O GlcNAc O GlcNAc transferase p110 subunit O GlcNAc transferase subunit p110 O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) O linked N acetylglucosamine (GlcNAc) transferase O linked N acetylglucosamine transferase 110 kDa subunit O-GlcNAc transferase subunit p110 O-linked N-acetylglucosamine transferase 110 kDa subunit ogt OGT1_HUMAN UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase |
Images
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Fig1:
Immunocytochemistry analysis of Hela cells labeling OGT with Rabbit anti-OGT antibody (HA720156F) at 1/75 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-OGT antibody (HA720156F) at 1/75 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.