iFluor™ 488 Conjugated CCR7 Recombinant Rabbit Monoclonal Antibody [SR36-04]
cat.: HA720157F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SR36-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CCR7 aa 13-62 / 378. |
| Positive control: | MCF-7, rat spleen tissue, human lymph nodes tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IF-Cell IF-Tissue |
1:100 1:50 |
| Uniprot #: | SwissProt: P32248 Human Entrez Gene: 287673 Rat Unigene: 229736 Rat |
| Alternative names: | BLR 2 BLR2 C C chemokine receptor type 7 C C CKR 7 C-C chemokine receptor type 7 C-C CKR-7 CC chemokine receptor 7 CC chemokine receptor type 7 CC CKR 7 CC-CKR-7 CCCKR7 CCR 7 CCR-7 Ccr7 CCR7_HUMAN CD 197 CD197 CD197 antigen CDw197 Chemokine (C C motif) receptor 7 Chemokine (C C) receptor 7 Chemokine C C motif receptor 7 Chemokine C C receptor 7 Chemokine receptor 7 Chemokine receptor 7-like protein CMKBR7 EBI 1 EBI1 Ebi1h EBV Induced G Protein Coupled Receptor 1 EBV-induced G-protein coupled receptor 1 Epstein Barr virus induced G protein coupled receptor Epstein Barr virus induced gene 1 Epstein-Barr virus-induced G-protein coupled receptor 1 EVI 1 EVI1 Lymphocyte Specific G Protein Coupled Peptide Receptor MGC108519 MIP 3 beta receptor MIP-3 beta receptor MIP3 Beta Receptor |
Images
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Fig1:
Immunocytochemistry analysis of MCF-7 cells labeling CCR7 with Rabbit anti-CCR7 antibody (HA720157F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-CCR7 antibody (HA720157F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution. |
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Fig2:
Immunofluorescence analysis of paraffin-embedded rat spleen tissue labeling CCR7 (HA720157F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody CCR7 (HA720157F, iFluor™ 488) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
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Fig3:
Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling CCR7 (HA720157F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody CCR7 (HA720157F, iFluor™ 488) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.