iFluor™ 594 Conjugated E-Cadherin Recombinant Rabbit Monoclonal Antibody [SY0287]
cat.: HA720164F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SY0287 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 97 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human E-Cadherin aa 591-640 / 882. |
| Positive control: | Human breast carcinoma tissue. |
| Subcellular location: | Endosome, Cell membrane, trans-Golgi network, adherens junction. |
| Recommended Dilutions:
IF-Tissue |
1:50 |
| Uniprot #: | SwissProt: P12830 Human |
| Alternative names: | Arc 1 CADH1_HUMAN Cadherin 1 cadherin 1 type 1 E-cadherin Cadherin1 CAM 120/80 CD 324 CD324 CD324 antigen cdh1 CDHE E-Cad/CTF3 E Cadherin E-cadherin ECAD Epithelial cadherin epithelial calcium dependant adhesion protein LCAM Liver cell adhesion molecule UVO Uvomorulin |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human breast carcinoma tissue labeling E-Cadherin (HA720164F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody E-Cadherin (HA720164F, iFluor™ 594) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.