iFluor™ 647 Conjugated Vimentin Recombinant Rabbit Monoclonal Antibody [SC60-05]
cat.: HA720165F
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: IF-Cell, IF-Tissue, FC
Clonality: Monoclonal
Clone number: SC60-05
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 54 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal human Vimentin.
Positive control: C2C12, Hela, L6, human kidney tissue, human pancreas tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  IF-Cell
  IF-Tissue
  FC

1:100
1:200
1:1,000
Uniprot #: SwissProt: P08670 Human | P20152 Mouse | P31000 Rat
Alternative names: CTRCT30 Epididymis luminal protein 113 FLJ36605 HEL113 VIM VIME_HUMAN Vimentin
Images
HA720165F_1.jpg Fig1: Immunocytochemistry analysis of C2C12 cells labeling Vimentin with Rabbit anti-Vimentin antibody (HA720165F) at 1/50 dilution.

Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (HA720165F) at 1/50 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, green) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution.
HA720165F_2.jpg Fig2: Immunocytochemistry analysis of Hela cells labeling Vimentin with Rabbit anti-Vimentin antibody (HA720165F) at 1/50 dilution.

Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (HA720165F) at 1/50 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, green) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution.
HA720165F_3.jpg Fig3: Immunocytochemistry analysis of L6 cells labeling Vimentin with Rabbit anti-Vimentin antibody (HA720165F) at 1/50 dilution.

Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (HA720165F) at 1/50 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, green) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution.
HA720165F_4.jpg Fig4: Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Vimentin (HA720165F).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Vimentin (HA720165F, iFluor™ 647) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.
HA720165F_5.jpg Fig5: Immunofluorescence analysis of paraffin-embedded human pancreas tissue labeling Vimentin (HA720165F).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Vimentin (HA720165F, iFluor™ 647) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.
HA720165F_6.jpg Fig6: Flow cytometric analysis of Hela cells labeling Vimentin.

Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Vimentin (HA720165F, iFluor™ 647, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.