iFluor™ 594 Conjugated NeuN Recombinant Rabbit Monoclonal Antibody [SR45-07]
cat.: HA720167F
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: IF-Tissue, IHC-Fr
Clonality: Monoclonal
Clone number: SR45-07
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 34 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human NeuN aa 20-60.
Positive control: Mouse brain tissue, mouse cerebral cortex tissue, mouse hippocampus tissue.
Subcellular location: Cytoplasm, Nucleus
Recommended Dilutions:
  IF-Tissue
  IHC-Fr

1:50
1:100
Uniprot #: SwissProt: A6NFN3 Human | Q8BIF2 Mouse
Unigene: 143966 Rat
Alternative names: FLJ56884 FLJ58356 Fox-1 homolog C fox1 homolog C Fox3 FOX3NeuN hexaribonucleotide binding protein 3 HRNBP3 NEUN neuronal nuclei Rbfox3 RFOX3_HUMAN RNA binding protein fox-1 homolog 3 RNA binding protein, fox 1 homolog (C. elegans) 3 hide
Images
HA720167F_1.jpg Fig1: Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NeuN (HA720167F).

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody NeuN (HA720167F, iFluor™ 594) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.
HA720167F_2.jpg Fig2: Immunofluorescence analysis of paraffin-embedded mouse cerebral cortex tissue labeling NeuN (HA720167F).

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody NeuN (HA720167F, iFluor™ 594) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.
HA720167F_3.jpg Fig3: Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling NeuN (HA720167F).

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody NeuN (HA720167F, iFluor™ 594) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.