iFluor™ 594 Conjugated NeuN Recombinant Rabbit Monoclonal Antibody [SR45-07]
cat.: HA720167F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Tissue, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | SR45-07 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human NeuN aa 20-60. |
| Positive control: | Mouse brain tissue, mouse cerebral cortex tissue, mouse hippocampus tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
IF-Tissue IHC-Fr |
1:50 1:100 |
| Uniprot #: | SwissProt: A6NFN3 Human | Q8BIF2 Mouse Unigene: 143966 Rat |
| Alternative names: | FLJ56884 FLJ58356 Fox-1 homolog C fox1 homolog C Fox3 FOX3NeuN hexaribonucleotide binding protein 3 HRNBP3 NEUN neuronal nuclei Rbfox3 RFOX3_HUMAN RNA binding protein fox-1 homolog 3 RNA binding protein, fox 1 homolog (C. elegans) 3 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NeuN (HA720167F). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody NeuN (HA720167F, iFluor™ 594) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
|
Fig2:
Immunofluorescence analysis of paraffin-embedded mouse cerebral cortex tissue labeling NeuN (HA720167F). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody NeuN (HA720167F, iFluor™ 594) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
|
Fig3:
Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling NeuN (HA720167F). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody NeuN (HA720167F, iFluor™ 594) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.