iFluor™ 594 Conjugated Heme Oxygenase 1 (HO-1) Recombinant Rabbit Monoclonal Antibody [SP08-07]
cat.: HA720169F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SP08-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HO-1 aa 227-276 / 288. |
| Positive control: | Human spleen tissue. |
| Subcellular location: | Microsome, Endoplasmic reticulum membrane. |
| Recommended Dilutions:
IF-Tissue |
1:50 |
| Uniprot #: | SwissProt: P09601 Human |
| Alternative names: | 32 kD bK286B10 D8Wsu38e heat shock protein 32 kD heat shock protein 32kD Heat shock protein Heme oxygenase (decycling) 1 Heme oxygenase 1 Hemox HMOX 1 Hmox Hmox1 HMOX1_HUMAN HO 1 HO HO-1 HO1 Hsp32 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human spleen tissue labeling Heme Oxygenase 1 (HO-1) (HA720169F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Heme Oxygenase 1 (HO-1) (HA720169F, iFluor™ 594) at 1/50 dilution overnight at 4 ℃, washed with PBS. 33258 was used as nuclear counterstain. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.