iFluor™ 488 Conjugated Lamin B1 Recombinant Rabbit Monoclonal Antibody [SI17-07]
cat.: HA720171F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SI17-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 66 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Lamin B1 aa 511-560 / 586. |
| Positive control: | Human colon carcinoma tissue, mouse colon tissue, rat colon tissue. |
| Subcellular location: | Nucleus inner membrane. |
| Recommended Dilutions:
IF-Tissue |
1:200 |
| Uniprot #: | SwissProt: P20700 Human | P14733 Mouse | P70615 Rat |
| Alternative names: | ADLD lamin B1 Lamin-B1 LMN LMN2 LMNB Lmnb1 LMNB1_HUMAN MGC111419 OTTHUMP00000159218 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling Lamin B1 with Rabbit anti-Lamin B1 antibody (HA720171F) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720171F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue). |
|
Fig2:
Immunofluorescence analysis of paraffin-embedded mouse colon tissue labeling Lamin B1 with Rabbit anti-Lamin B1 antibody (HA720171F) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720171F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue). |
|
Fig3:
Immunofluorescence analysis of paraffin-embedded rat colon tissue labeling Lamin B1 with Rabbit anti-Lamin B1 antibody (HA720171F) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720171F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.