Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Mouse, Rat |
Applications: | IF-Tissue, FC |
Clonality: | Monoclonal |
Clone number: | JM52-44 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 83 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human SATB2 238-287 / 733. |
Positive control: | Human colon carcinoma tissue, THP-1. |
Subcellular location: | Nucleus matrix. |
Recommended Dilutions:
IF-Tissue FC |
1:200 1:500-1:1,000 |
Uniprot #: | SwissProt: Q9UPW6 Human |
Alternative names: | DNA binding protein SATB2 DNA-binding protein SATB2 FLJ21474 FLJ32076 GLSS KIAA1034 MGC119474 MGC119477 SATB family member 2 SATB homeobox 2 SATB2 SATB2_HUMAN Special AT rich sequence binding protein 2 Special AT-rich sequence-binding protein 2 |
Fig1:
Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling SATB2 (HA720173F). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody SATB2 (HA720173F, iFluor™ 647) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
|
Fig2:
Flow cytometric analysis of THP-1 cells labeling SATB2. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with SATB2 (HA720173F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |