iFluor™ 647 Conjugated SATB2 Recombinant Rabbit Monoclonal Antibody [JM52-44]
cat.: HA720173F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | JM52-44 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 83 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SATB2 238-287 / 733. |
| Positive control: | Human colon carcinoma tissue, THP-1. |
| Subcellular location: | Nucleus matrix. |
| Recommended Dilutions:
IF-Tissue FC |
1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9UPW6 Human |
| Alternative names: | DNA binding protein SATB2 DNA-binding protein SATB2 FLJ21474 FLJ32076 GLSS KIAA1034 MGC119474 MGC119477 SATB family member 2 SATB homeobox 2 SATB2 SATB2_HUMAN Special AT rich sequence binding protein 2 Special AT-rich sequence-binding protein 2 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling SATB2 (HA720173F). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody SATB2 (HA720173F, iFluor™ 647) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
|
Fig2:
Flow cytometric analysis of THP-1 cells labeling SATB2. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with SATB2 (HA720173F, red, 1/1,000) and Rabbit IgG Isotype Control (iFluor™ 647, green, 1/1,000). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.