iFluor™ 647 Conjugated Sodium Potassium ATPase Recombinant Rabbit Monoclonal Antibody [ST0533]
cat.: HA720176F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | ST0533 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 100 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ATP1A1 aa 39-83 / 1023. |
| Positive control: | Rat kidney tissue, human liver tissue, Hela. |
| Subcellular location: | Cell membrane, Melanosome. |
| Recommended Dilutions:
IF-Tissue FC |
1:100 1:500-1:1,000 |
| Uniprot #: | SwissProt: P05023 Human | P05026 Human | Q13733 Human | P06685 Rat | P07340 Rat | Q64541 Rat |
| Alternative names: | ATPase Na+/K+ transporting alpha adenosinetriphosphatase AT1A1_HUMAN ATP1A1 ATP1A4 ATP1AL2 ATP1B ATP1B1 ATPase Na+/K+ transporting alpha 1 polypeptide ATPase Na+/K+ transporting alpha 4 polypeptide ATPase Na+/K+ transporting beta 1 polypeptide ATPase, Na+/K+ transporting, alpha polypeptide-like 2 ATPase, Na+/K+ transporting, beta 1 polypeptide Beta 1-subunit of Na(+),K(+)-ATPase Na(+)/K(+) ATPase alpha-1 subunit Na(+)/K(+) ATPase alpha-4 subunit Na+, K+ ATPase alpha subunit Na+/K+ ATPase 1 Na+/K+ ATPase 4 Na+/K+ ATPase, alpha-D polypeptide Na, K-ATPase beta-1 polypeptide Na, K-ATPase, alpha-A catalytic polypeptide Na,K-ATPase catalytic subunit alpha-A protein Na,K-ATPase subunit alpha-C polypeptide-like 2 Sodium pump 1 sodium pump 4 Sodium pump subunit alpha-1 sodium pump subunit alpha-4 sodium pump subunit beta-1 sodium-potassium ATPase catalytic subunit alpha-1 sodium-potassium ATPase catalytic subunit alpha-4 sodium-pot...... |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded rat kidney tissue labeling Sodium Potassium ATPase (HA720176F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Sodium Potassium ATPase (HA720176F, iFluor™ 647) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
|
Fig2:
Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Sodium Potassium ATPase (HA720176F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Sodium Potassium ATPase (HA720176F, iFluor™ 647) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. |
|
Fig3:
Flow cytometric analysis of Hela cells labeling Sodium Potassium ATPase. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Sodium Potassium ATPase (HA720176F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.