iFluor™ 488 Conjugated NSE Recombinant Rabbit Monoclonal Antibody [SC06-28]
cat.: HA720178F
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: IF-Cell, FC
Clonality: Monoclonal
Clone number: SC06-28
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 47 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human NSE aa 30-70.
Positive control: SH-SY5Y.
Subcellular location: Cytoplasm, Cell membrane.
Recommended Dilutions:
  IF-Cell
  FC

1:50
1:500-1:1,000
Uniprot #: SwissProt: P09104 Human | P17183 Mouse | P07323 Rat
Alternative names: 2 phospho D glycerate hydrolyase 2-phospho-D-glycerate hydro-lyase Eno 2 ENO2 ENOG ENOG_HUMAN Enolase 2 (gamma, neuronal) Enolase 2 Enolase 2 gamma neuronal Enolase2 Epididymis secretory protein Li 279 Gamma enolase Gamma-enolase HEL S 279 Neural enolase Neuron specific enolase Neuron specific gamma enolase Neuron-specific enolase Neurone specific enolase NSE
Images
HA720178F_1.jpg Fig1: Immunocytochemistry analysis of SH-SY5Y cells labeling NSE with Rabbit anti-NSE antibody (HA720178F) at 1/50 dilution.

Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NSE antibody (HA720178F) at 1/50 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
HA720178F_2.jpg Fig2: Flow cytometric analysis of SH-SY5Y cells labeling NSE.

Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with NSE (HA720178F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.