Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Mouse, Rat |
Applications: | IF-Cell, FC |
Clonality: | Monoclonal |
Clone number: | SC06-28 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 47 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human NSE aa 30-70. |
Positive control: | SH-SY5Y. |
Subcellular location: | Cytoplasm, Cell membrane. |
Recommended Dilutions:
IF-Cell FC |
1:50 1:500-1:1,000 |
Uniprot #: | SwissProt: P09104 Human | P17183 Mouse | P07323 Rat |
Alternative names: | 2 phospho D glycerate hydrolyase 2-phospho-D-glycerate hydro-lyase Eno 2 ENO2 ENOG ENOG_HUMAN Enolase 2 (gamma, neuronal) Enolase 2 Enolase 2 gamma neuronal Enolase2 Epididymis secretory protein Li 279 Gamma enolase Gamma-enolase HEL S 279 Neural enolase Neuron specific enolase Neuron specific gamma enolase Neuron-specific enolase Neurone specific enolase NSE |
Fig1:
Immunocytochemistry analysis of SH-SY5Y cells labeling NSE with Rabbit anti-NSE antibody (HA720178F) at 1/50 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NSE antibody (HA720178F) at 1/50 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. |
|
Fig2:
Flow cytometric analysis of SH-SY5Y cells labeling NSE. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with NSE (HA720178F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |