iFluor™ 488 Conjugated SOX2 Recombinant Rabbit Monoclonal Antibody [PO00-28]
cat.: HA720179F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PO00-28 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SOX2 aa 1-100 (N terminal). |
| Positive control: | F9 cell. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IF-Cell FC |
1:50 1ug/mL |
| Uniprot #: | SwissProt: P48431 Human | P48432 Mouse |
| Alternative names: | ANOP3 cb236 Delta EF2a lcc MCOPS3 MGC148683 MGC2413 RGD1565646 Sex determining region Y box 2 SOX 2 Sox2 SOX2_HUMAN SRY (sex determining region Y) box 2 SRY box containing gene 2 SRY related HMG box 2 SRY related HMG box gene 2 SRY-box 2 Transcription factor SOX 2 Transcription factor SOX-2 ysb |
Images
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Fig1:
Immunocytochemistry analysis of F9 cells labeling SOX2 with Rabbit anti-SOX2 antibody (HA720179F) at 1/50 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SOX2 antibody (HA720179F) at 1/50 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. |
|
Fig2:
Flow cytometric analysis of F9 cells labeling SOX2. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with SOX2 (HA720179F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.