iFluor™ 647 Conjugated EpCAM Recombinant Rabbit Monoclonal Antibody [PS01-69]
cat.: HA720185F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | FC, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PS01-69 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide. |
| Positive control: | HT-29. |
| Subcellular location: | Lateral cell membrane, Cell junction. |
| Recommended Dilutions:
FC IF-Cell |
1:1,000 1:500 |
| Uniprot #: | SwissProt: P16422 Human |
| Alternative names: | 17 1A 323/A3 Adenocarcinoma associated antigen Adenocarcinoma-associated antigen Antigen identified by monoclonal AUA1 AUA1 CD326 CD326 antigen Cell surface glycoprotein Trop 1 Cell surface glycoprotein Trop 2 Cell surface glycoprotein Trop-1 CO 17A CO17 1A CO17A DIAR5 EGP 2 EGP EGP2 EGP314 EGP40 Ep CAM Ep-CAM EPCAM EPCAM_HUMAN EpCAM1 Epithelial cell adhesion molecule Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD) Epithelial cell surface antigen Epithelial cellular adhesion molecule Epithelial glycoprotein 1 Epithelial glycoprotein 314 Epithelial glycoprotein ESA GA733 1 GA733 2 GA733-2 gastrointestinal tumor-associated antigen 2, 35-KD glycoprotein gp4 hEGP 2 hEGP314 HNPCC8 Human epithelial glycoprotein 2 KS 1/4 antigen KS1/4 KSA Ly74 Lymphocyte antigen 74 M1S 1 M1S2 M4S1 Major gastrointestinal tumor associated protein GA733 2 Major gastrointestinal tumor-associa...... |
Images
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Fig1:
Flow cytometric analysis of HT-29 cells labeling EpCAM (HA720185F, iFluor™ 647). Cells were washed twice with cold PBS and resuspend. Then incubated for 1 hour at +4℃ with EpCAM (HA720185F, red, 1/1,000) and Rabbit IgG Isotype Control (iFluor™ 647, green, 1/1,000). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig2:
Immunocytochemistry analysis of HT-29 cells labeling EpCAM with Rabbit anti-EpCAM antibody (HA720185F) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-EpCAM antibody (HA720185F, iFluor™ 647) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.