iFluor™ 647 Conjugated BrdU Recombinant Rabbit Monoclonal Antibody [PSH0-18]
cat.: HA720188F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH0-18 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | BrdU-OVA |
| Positive control: | BrdU treated mouse embryo tissue, BrdU treated HeLa. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IF-Tissue FC |
1:200 1:20 |
| Alternative names: | Bromodeoxyuridine BUdr |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded BrdU treated/untreated mouse embryo tissue labeling BrdU with Rabbit anti-BrdU antibody (HA720188F) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720188F, red) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue). |
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Fig2:
Dot plot showing BrdU treated HeLa cells stained with HA720188F. Cells were incubated with 10 µM BrdU for 24 hours prior to being harvested, washed twice in 1x PBS and fixed in 70% ethanol at 4℃ for 30 minutes. Once fixed, pellets were acid denatured with 2M HCl for 30 minutes at room temperature and then neutralised with borate buffer (0.1M, pH8.5) for 15 minutes. Samples were washed and incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (HA720188F, 50µg/ml) for 30 min at room temperature. PI was added to cells 15 min prior to data acquisition. |
|
Fig3:
Dot plot showing untreated HeLa cells (Negative) stained with HA720188F. Cells were incubated without 10 µM BrdU for 30 minutes prior to being harvested, washed twice in 1x PBS and fixed in 70% ethanol at 4℃ for 30 minutes. Once fixed, pellets were acid denatured with 2M HCl for 30 minutes at room temperature and then neutralised with borate buffer (0.1M, pH8.5) for 15 minutes. Samples were washed and incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (HA720188F, 50µg/ml) for 30 min at room temperature. PI was added to cells 15 min prior to data acquisition. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.