iFluor™ 488 Conjugated p75 NGF Receptor Recombinant Rabbit Monoclonal Antibody [SA39-02]
cat.: HA720190F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | SA39-02 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human p75 NGF Receptor aa 345-394 / 427. |
| Positive control: | PC-12. |
| Subcellular location: | Membrane |
| Recommended Dilutions:
IF-Cell |
1:100 |
| Uniprot #: | SwissProt: P08138 Human | Q9Z0W1 Mouse | P07174 Rat |
| Alternative names: | CD271 CD271 antigen Gp80 LNGFR Gp80-LNGFR Low affinity nerve growth factor receptor Low affinity neurotrophin receptor p75NTR Low-affinity nerve growth factor receptor Nerve growth factor receptor Nerve growth factor receptor TNFR superfamily member 16 NGF receptor Ngfr p75 ICD p75 Neurotrophin receptor p75 NTR p75(NTR) p75NTR TNFR Superfamily Member 16 TNFRSF16 TNR16_HUMAN Tumor necrosis factor receptor superfamily member 16 |
Images
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Fig1:
Immunocytochemistry analysis of PC-12 cells labeling p75 NGF Receptor with Rabbit anti-p75 NGF Receptor antibody (HA720190F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p75 NGF Receptor antibody (HA720190F) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.