| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | FC |
| Clonality: | Monoclonal |
| Clone number: | JF104-8 |
| Form: | Liquid |
| Storage condition: | Store at 2℃ to 8℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA. |
| Concentration: | / |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Bcl-2 aa 1-230. |
| Positive control: | Mouse granulocytes, mouse lymphocytes. |
| Subcellular location: | Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm. |
| Recommended Dilutions:
FC |
5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. |
| Uniprot #: | SwissProt: P10415 Human | P10417 Mouse |
| Alternative names: | Apoptosis regulator Bcl 2 Apoptosis regulator Bcl-2 Apoptosis regulator Bcl2 AW986256 B cell CLL/lymphoma 2 B cell leukemia/lymphoma 2 Bcl-2 Bcl2 BCL2_HUMAN C430015F12Rik D630044D05Rik D830018M01Rik Leukemia/lymphoma, B-cell, 2 Oncogene B-cell leukemia 2 PPP1R50 Protein phosphatase 1, regulatory subunit 50 Bcl 2 |
|
Fig1:
Flow cytometric analysis of mouse granulocytes labeling Bcl-2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA720210F, 1μg/mL) (red). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig2:
Flow cytometric analysis of mouse lymphocytes labeling Bcl-2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA720210F, 1μg/mL) (red). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |