Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | FC |
Clonality: | Monoclonal |
Clone number: | JF104-8 |
Form: | Liquid |
Storage condition: | Store at 2℃ to 8℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA. |
Concentration: | / |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 26 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human Bcl-2 aa 1-230. |
Positive control: | Mouse granulocytes, mouse lymphocytes. |
Subcellular location: | Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm. |
Recommended Dilutions:
FC |
5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. |
Uniprot #: | SwissProt: P10415 Human | P10417 Mouse |
Alternative names: | Apoptosis regulator Bcl 2 Apoptosis regulator Bcl-2 Apoptosis regulator Bcl2 AW986256 B cell CLL/lymphoma 2 B cell leukemia/lymphoma 2 Bcl-2 Bcl2 BCL2_HUMAN C430015F12Rik D630044D05Rik D830018M01Rik Leukemia/lymphoma, B-cell, 2 Oncogene B-cell leukemia 2 PPP1R50 Protein phosphatase 1, regulatory subunit 50 Bcl 2 |
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Fig1:
Flow cytometric analysis of mouse granulocytes labeling Bcl-2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA720210F, 1μg/mL) (red). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig2:
Flow cytometric analysis of mouse lymphocytes labeling Bcl-2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA720210F, 1μg/mL) (red). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |