FITC Conjugated 6X His tag Recombinant Rabbit Monoclonal Antibody [PSH07-10]
cat.: HA720222F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH07-10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide immune sequence is HHHHHHHHC. |
| Recommended Dilutions:
IF-Cell FC |
1:1,000 1:1,000 |
| Alternative names: | 6 His epitope tag Hexa His tag HHHHHH epitope tag HHHHHH tag His tag Polyhistidine Tag |
Images
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Fig1:
Immunocytochemistry analysis of HeLa cells labeling 6X His tag with Rabbit anti-6X His tag antibody (HA720222F) at 1/1,000 dilution. HeLa cells, transfected with empty control (top, negative) / His-tagged Histone H3.1 (middle, positive) / His-tagged ACAT2 (bottom, positive) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-6X His tag antibody (HA720222F, FITC, green) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig2:
Flow cytometric analysis of HeLa cells (left) / HeLa cells transfected with His-tagged ACAT2 (right) labeling 6X His tag (HA720222F, FITC). Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with 6X His tag (HA720222F, FITC, red, 1/1,000) and Rabbit IgG Isotype Control (FITC, green, 1/1,000). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.