iFluor™ 594 Conjugated HA tag Recombinant Rabbit Monoclonal Antibody [PSH10-66]
cat.: HA720224F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH10-66 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N Terminal fusion protein and C Terminal fusion protein. |
| Recommended Dilutions:
IF-Cell FC |
1:100 1:1,000 |
| Alternative names: | HA epitope tag HA1 HA2 hemagglutinin Hemagglutinin HA1 chain Hemagglutinin HA2 chain |
Images
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Fig1:
Immunocytochemistry analysis of 293T cells labeling HA tag with Rabbit anti-HA tag antibody (HA720224F) at 1/100 dilution. 293T cells, transfected with empty control (top, negative) / HA-tagged CLK4 (middle, positive) / HA-tagged Nanos homolog 3 (bottom, positive) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HA tag antibody (HA720224F, iFluor™ 594, red) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. |
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Fig2:
Flow cytometric analysis of 293T cells (left) / 293T cells transfected with HA-tagged CLK4 (right) labeling HA tag (HA720224F, iFluor™ 594). Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with HA tag (HA720224F, iFluor™ 594, red, 1/1,000) and Rabbit IgG Isotype Control (iFluor™ 594, green, 1/1,000). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.