FITC Conjugated V5 tag Recombinant Rabbit Monoclonal Antibody [SY30-01]
cat.: HA720227F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | SY30-01 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide immune sequence is CGKPIPNPLLGLDST. |
| Recommended Dilutions:
IF-Cell |
1:1,000 |
| Alternative names: | GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST tag Protein Rev Regulator of expression of viral proteins rev V5 epitope tag |
Images
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Fig1:
Immunocytochemistry analysis of HeLa cells labeling V5 tag with Rabbit anti-V5 tag antibody (HA720227F) at 1/1,000 dilution. HeLa cells, untransfected (top, negative) / transfected with V5-tagged Histone H3.1 (bottom, positive) expression vector, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-V5 tag antibody (HA720227F, FITC, green) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Histone H3 (HA601278, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.