BIN1 Recombinant Rabbit Monoclonal Antibody [JE34-92]
cat.: HA721001
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE34-92 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 65 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human BIN1 aa 394-593/593. |
| Positive control: | SiHa cell lysates, rat skeletal muscle tissue, human colon carcinoma tissue, human kidney tissue, SiHa. |
| Subcellular location: | Cytoplasm, T-tubule, Endosome, Nucleus; Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:50 1:400 |
| Uniprot #: | SwissProt: O00499 Human | O08839 Rat |
| Alternative names: | AMPH 2 AMPH2 Amphiphysin 2 Amphiphysin II Amphiphysin like protein amphiphysin-like Amphiphysin-like protein AMPHL Bin1 BIN1_HUMAN Box Dependant MYC Interacting Protein 1 Box-dependent myc-interacting protein 1 Bridging integrator 1 DKFZp547F068 MGC10367 MGC105358 Myc box dependent interacting protein 1 Myc box-dependent-interacting protein 1 SH3P9 |
Images
|
Fig1:
Western blot analysis of BIN1 on SiHa cell lysates with Rabbit anti-BIN1 antibody (HA721001) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 65 kDa Observed band size: 60/50 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721001) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-BIN1 antibody (HA721001) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721001) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-BIN1 antibody (HA721001) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721001) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-BIN1 antibody (HA721001) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721001) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunocytochemistry analysis of SiHa cells labeling BIN1 with Rabbit anti-BIN1 antibody (HA721001) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-BIN1 antibody (HA721001) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.