TCEB2 Recombinant Rabbit Monoclonal Antibody [JE65-72]
cat.: HA721002
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE65-72 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 13 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human TCEB2 aa 1-50/118. |
| Positive control: | Mouse testis tissue lysate, Rat testis tissue lysate, Hela cell lysate, Human lung tissue lysate, human testis tissue, human thyroid tissue, human breast tissue, human breast carcinoma tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:5,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: Q15370 Human | P62869 Mouse | P62870 Rat |
| Alternative names: | EloB ELOB_HUMAN Elongin 18 kDa subunit Elongin B Elongin-B RNA polymerase II transcription factor SIII p18 subunit RNA polymerase II transcription factor SIII subunit B SIII SIII p18 TCEB 2 Tceb2 Transcription elongation factor B (SIII) polypeptide 2 Transcription elongation factor B polypeptide 2 |
Images
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Fig1:
Western blot analysis of TCEB2 on different lysates with Rabbit anti-TCEB2 antibody (HA721002) at 1/5,000 dilution. Lane 1: Mouse testis tissue lysate Lane 2: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 13 kDa Observed band size: 15 kDa Exposure time: 35 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721002) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of TCEB2 on different lysates with Rabbit anti-TCEB2 antibody (HA721002) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Human lung tissue lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 13 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721002) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TCEB2 antibody (HA721002) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721002) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-TCEB2 antibody (HA721002) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721002) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-TCEB2 antibody (HA721002) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721002) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-TCEB2 antibody (HA721002) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721002) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.