CLIP170 Recombinant Rabbit Monoclonal Antibody [JE63-82]
cat.: HA721004
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE63-82 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 162 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CLIP170 aa 1,389-1,438/1,438. |
| Positive control: | Hela cell lysate, HepG2 cell lysate, MCF-7 cell lysate, HUVEC cell lysate, JAR cell lysate, human skin tissue, Hela. |
| Subcellular location: | Cytoskeleton, cytoplasm, cytoplasmic vesicle membrane, ruffle. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:50 1:100 |
| Uniprot #: | SwissProt: P30622 Human |
| Alternative names: | CAP GLY domain containing linker protein 1 CAP-Gly domain-containing linker protein 1 CLIP 170 CLIP CLIP-170 CLIP1 CLIP1_HUMAN CYLN1 cytoplasmic linker 1 Cytoplasmic linker protein 1 Cytoplasmic linker protein 170 alpha 2 Cytoplasmic linker protein 170 alpha-2 Cytoplasmic linker protein CLIP 170 Reed Steinberg cell expressed intermediate filament associated Reed Sternberg intermediate filament associated protein 3 Reed-Sternberg intermediate filament-associated protein Restin RSN |
Images
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Fig1:
Western blot analysis of CLIP170 on different lysates with Rabbit anti-CLIP170 antibody (HA721004) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: MCF-7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 162 kDa Observed band size: 162 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721004) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of CLIP170 on different lysates with Rabbit anti-CLIP170 antibody (HA721004) at 1/500 dilution. Lane 1: HUVEC cell lysate Lane 2: JAR cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 162 kDa Observed band size: 162 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721004) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-CLIP170 antibody (HA721004) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721004) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of Hela cells labeling CLIP170 with Rabbit anti-CLIP170 antibody (HA721004) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CLIP170 antibody (HA721004) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.