GNA13 Recombinant Rabbit Monoclonal Antibody [JE63-76]
cat.: HA721007
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE63-76 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GNA13 aa 278-377/377. |
| Positive control: | 293T cell lysate, HepG2 cell lysate, 293 cell lysate, Jurkat cell lysate, PC-12 cell lysate, NIH/3T3 cell lysates, mouse colon tissue. |
| Subcellular location: | Cell membrane, Nucleus, Cytoplasm, Melanosome. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:500 |
| Uniprot #: | SwissProt: Q14344 Human | P27601 Mouse | Q6Q7Y5 Rat |
| Alternative names: | G alpha 13 G alpha-13 G-protein subunit alpha-13 GNA13 GNA13_HUMAN Guanine nucleotide binding protein (G protein) alpha 13 Guanine nucleotide binding protein alpha 13 subunit Guanine nucleotide-binding protein subunit alpha-13 |
Images
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Fig1:
Western blot analysis of GNA13 on different lysates with Rabbit anti-GNA13 antibody (HA721007) at 1/500 dilution. Lane 1: 293T cell lysate Lane 2: HepG2 cell lysate Lane 3: 293 cell lysate Lane 4: Jurkat cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 44 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721007) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of GNA13 on NIH/3T3 cell lysates with Rabbit anti-GNA13 antibody (HA721007) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 44 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721007) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-GNA13 antibody (HA721007) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721007) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.