SLP-2 Recombinant Rabbit Monoclonal Antibody [JE63-15]
cat.: HA721009
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE63-15 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SLP-2 aa 1-200/356. |
| Positive control: | Hela cell lysate, Jurkat cell lysate, Rat brain tissue lysate, Mouse spleen tissue lysate, NIH/3T3 cell lysate, Rat spleen tissue lysate, HeLa, human kidney tissue, rat testis tissue, HepG2. |
| Subcellular location: | Mitochondrion, Mitochondrion inner membrane, Mitochondrion intermembrane space, Cytoskeleton, Cell membrane, Membrane raft. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:1,000 1:400-1:1,000 1:50 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9UJZ1 Human | Q99JB2 Mouse | Q4FZT0 Rat |
| Alternative names: | EPB72 like protein 2 EPB72-like protein 2 HSPC108 OTTHUMP00000021320 Paraprotein target 7 Paratarg 7 SLP 2 SLP-2 SLP2 STML2_HUMAN Stomatin (EPB72) like 2 Stomatin like 2 Stomatin like protein 2 Stomatin like protein 2, mitochondrial Stomatin-like protein 2 STOML2 |
Images
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Fig1:
Western blot analysis of SLP-2 on different lysates with Rabbit anti-SLP-2 antibody (HA721009) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-SLP-2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 39 kDa Observed band size: 39 kDa Exposure time: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721009) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of SLP-2 on different lysates with Rabbit anti-SLP-2 antibody (HA721009) at 1/1,000 dilution. Lane 1: Hela cell lysate, 10 µg/Lane Lane 2: Jurkat cell lysate, 10 µg/Lane Lane 3: Rat brain tissue lysate, 20 µg/Lane Lane 4: Mouse spleen tissue lysate, 20 µg/Lane Lane 5: NIH/3T3 cell lysate, 10 µg/Lane Lane 6: Rat spleen tissue lysate, 20 µg/Lane Predicted band size: 39 kDa Observed band size: 39 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721009) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of HeLa cells labeling SLP-2 with Rabbit anti-SLP-2 antibody (HA721009) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SLP-2 antibody (HA721009) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SLP-2 antibody (HA721009) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721009) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-SLP-2 antibody (HA721009) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721009) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Flow cytometric analysis of HepG2 cells labeling SLP-2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721009, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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