SLP-2 Recombinant Rabbit Monoclonal Antibody [JE63-15]
cat.: HA721009
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JE63-15
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 39 kDa
Isotype: IgG
Immunogen: Recombinant protein within human SLP-2 aa 1-200/356.
Positive control: Hela cell lysate, Jurkat cell lysate, rat brain tissue lysate, mouse spleen tissue lysate, NIH/3T3 cell lysate, rat spleen tissue lysate, rat testis tissue, human kidney tissue, HepG2.
Subcellular location: Mitochondrion, Mitochondrion inner membrane, Mitochondrion intermembrane space, Cytoskeleton, Cell membrane, Membrane raft.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:1,000
1:400
1:500-1:1,000
Uniprot #: SwissProt: Q9UJZ1 Human | Q99JB2 Mouse | Q4FZT0 Rat
Alternative names: EPB72 like protein 2 EPB72-like protein 2 HSPC108 OTTHUMP00000021320 Paraprotein target 7 Paratarg 7 SLP 2 SLP-2 SLP2 STML2_HUMAN Stomatin (EPB72) like 2 Stomatin like 2 Stomatin like protein 2 Stomatin like protein 2, mitochondrial Stomatin-like protein 2 STOML2
Images
HA721009_1.jpg Fig1: Western blot analysis of SLP-2 on different lysates with Rabbit anti-SLP-2 antibody (HA721009) at 1/500 dilution.

Lane 1: Hela cell lysate, 10 µg/Lane
Lane 2: Jurkat cell lysate, 10 µg/Lane
Lane 3: Rat brain tissue lysate, 20 µg/Lane
Lane 4: Mouse spleen tissue lysate, 20 µg/Lane
Lane 5: NIH/3T3 cell lysate, 10 µg/Lane
Lane 6: Rat spleen tissue lysate, 20 µg/Lane

Predicted band size: 39 kDa
Observed band size: 39 kDa

Exposure time: 1 minute;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721009) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA721009_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-SLP-2 antibody (HA721009) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721009) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721009_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SLP-2 antibody (HA721009) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721009) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721009_4.jpg Fig4: Flow cytometric analysis of HepG2 cells labeling SLP-2.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721009, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.