Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE63-23 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 39 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human P2Y12 aa 101-342/342. |
Positive control: | NIH/3T3 cell lysate, PC-3 cell lysate. |
Subcellular location: | Cell membrane. |
Recommended Dilutions:
WB |
1:500 |
Uniprot #: | SwissProt: Q9H244 Human | Q9CPV9 Mouse |
Alternative names: | ADP glucose receptor ADP-glucose receptor ADPG R ADPG-R ADPGR BDPLT8 G protein coupled receptor SP1999 Gi coupled ADP receptor HORK 3 Gi coupled ADP receptor HORK3 HORK 3 HORK3 P2RY 12 P2RY12 P2T(AC) P2Y 12 P2Y purinoceptor 12 P2Y(12)R P2Y(AC) P2Y(ADP) P2Y(cyc) P2Y12 P2Y12 platelet ADP receptor P2Y12_HUMAN Platelet ADP receptor Purinergic receptor P2RY12 Purinergic receptor P2Y G protein coupled 12 Purinergic receptor P2Y12 Putative G protein coupled receptor SP 1999 SP1999 |
Fig1:
Western blot analysis of P2Y12 on different lysates with Rabbit anti-P2Y12 antibody (HA721011) at 1/500 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: PC-3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 39 kDa Observed band size: 37 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721011) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |