| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE63-23 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human P2Y12 aa 101-342/342. |
| Positive control: | NIH/3T3 cell lysate, PC-3 cell lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: Q9H244 Human | Q9CPV9 Mouse |
| Alternative names: | ADP glucose receptor ADP-glucose receptor ADPG R ADPG-R ADPGR BDPLT8 G protein coupled receptor SP1999 Gi coupled ADP receptor HORK 3 Gi coupled ADP receptor HORK3 HORK 3 HORK3 P2RY 12 P2RY12 P2T(AC) P2Y 12 P2Y purinoceptor 12 P2Y(12)R P2Y(AC) P2Y(ADP) P2Y(cyc) P2Y12 P2Y12 platelet ADP receptor P2Y12_HUMAN Platelet ADP receptor Purinergic receptor P2RY12 Purinergic receptor P2Y G protein coupled 12 Purinergic receptor P2Y12 Putative G protein coupled receptor SP 1999 SP1999 |
|
Fig1:
Western blot analysis of P2Y12 on NIH/3T3 cell lysate with Rabbit anti-P2Y12 antibody (HA721011) at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721011, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 39.4 kDa Observed band size: 50 kDa |