P2Y12 Recombinant Rabbit Monoclonal Antibody [JE63-23]
cat.: HA721011
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB
Clonality: Monoclonal
Clone number: JE63-23
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 39 kDa
Isotype: IgG
Immunogen: Recombinant protein within human P2Y12 aa 101-342/342.
Positive control: NIH/3T3 cell lysate, PC-3 cell lysate.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB

1:500
Uniprot #: SwissProt: Q9H244 Human | Q9CPV9 Mouse
Alternative names: ADP glucose receptor ADP-glucose receptor ADPG R ADPG-R ADPGR BDPLT8 G protein coupled receptor SP1999 Gi coupled ADP receptor HORK 3 Gi coupled ADP receptor HORK3 HORK 3 HORK3 P2RY 12 P2RY12 P2T(AC) P2Y 12 P2Y purinoceptor 12 P2Y(12)R P2Y(AC) P2Y(ADP) P2Y(cyc) P2Y12 P2Y12 platelet ADP receptor P2Y12_HUMAN Platelet ADP receptor Purinergic receptor P2RY12 Purinergic receptor P2Y G protein coupled 12 Purinergic receptor P2Y12 Putative G protein coupled receptor SP 1999 SP1999
Images
HA721011_1.jpg Fig1: Western blot analysis of P2Y12 on different lysates with Rabbit anti-P2Y12 antibody (HA721011) at 1/500 dilution.

Lane 1: NIH/3T3 cell lysate
Lane 2: PC-3 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 39 kDa
Observed band size: 37 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721011) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.